FASTA Validator

Validate FASTA format and check sequence integrity

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Validation Results

FASTA Format Validator for Sequence Files

Our free FASTA validator checks FASTA format compliance and validates sequence integrity, ensuring your FASTA files are correctly formatted for bioinformatics tools and databases. The FASTA format checker identifies common errors including missing headers, invalid characters, and sequence format issues that could cause problems in downstream analysis.

The FASTA validator parses your input to verify it follows the standard FASTA format with proper header lines (starting with ">") and sequence data. It checks for sequence validation errors, invalid characters, and provides detailed feedback on any issues found. This tool is essential for researchers preparing sequences for submission to databases like GenBank, NCBI, or UniProt. Use our main sequence analyzer for comprehensive analysis, or our GC content calculator and ORF finder for additional sequence analysis capabilities.

All FASTA validation is performed instantly in your browser with complete privacy. The FASTA format validator supports both DNA and protein sequences, making it a versatile tool for molecular biologists, bioinformatics researchers, and students working with sequence analysis and database submissions.

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